The state says a 73-year-old Louisville man has died of West Nile virus.
Health and Family Services Cabinet spokeswoman Beth Fisher says it is Kentucky's first death from the virus reported this year.
The state Health Department says Jefferson County typically reports a few cases of the virus each year, mostly during late summer and early fall.
The Clark County Health Department in southern Indiana, across the Ohio River from Louisville, identified two samples of mosquitoes infected with the virus this week in Charlestown and Jeffersonville.
The cabinet advises wearing bug repellent and protective clothing outdoors and staying indoors at dawn, dusk and early evening when mosquitoes are most active.
The cabinet's website says symptoms of West Nile may include a slight fever or headache, possibly with skin rash and swollen lymph glands.